GAPDH Antibody: G3PD, GAPD, HEL-S-162eP, CDABP0047, OK/SW-cl. Antibodies should not be exposed to prolonged high temperatures. Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. All other applications and species not yet tested. Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. If you are blotting for CD63, CD81, CD9 marker, you should avoid reducing agents like DTT or BME because disulfide bonds are required for antibody to recognize epitope of these tetraspanine. GAPDH antibody can be used for the detection of GAPDH by Western blot at 1 μg/mL. GAPDH Western Blot Most recent answer Mateo Glumac University of Split 15th Feb, 2021 I found some westerns on the internet with double bands for GAPDH in Rat and Mouse brain homogenate but. Buy GAPDH enzyme, Glyceraldehyde 3-Phosphate Dehydrogenase, Rabbit, Western Blot Control Enzyme-NP001075722.1 (MBS653888) product datasheet at MyBioSource. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. Simple Western analysis of lysates (0.1 mg/mL) from HeLa cells using GAPDH (D16H11) XP Rabbit mAb 5174. Anti-Myc and GAPDH monoclonal antibodies were purchased from Santa Cruz Biotechnology. Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP Rabbit mAb. GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human GAPDH.The immunogen is located within amino acids 230 - 280 of GAPDH. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and. Sheep, Gallus, Rabbit, Bovine, Porcine, Guinea pig 10 A second, unrelated protein that is common to all samples (often a housekeeping protein such as actin, tubulin, or GAPDH) is typically detected on the same.
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